%0 Journal Article
%T Conjugation and Immunogenic Evaluation of Complete Immunogen for the Small Molecular Environmental Pollutants 2,4-D<br>2,4-D完全抗原的合成及其免疫性能评价
%A YU Ruo-zhen
%A HE Miao
%A SHI Han-chang
%A LIU Ju
%A GUO Jie-han
%A WANG Na
%A QIAN Yi
%A <br>余若祯
%A 何苗
%A 施汉昌
%A 刘举
%A 郭洁菡
%A 王娜
%A 钱易
%J 环境科学
%D 2006
%I 
%X Conjugation of complete immunogen for 2,4-dichlorophenoxyacetic acid (2,4-D) was studied. 2,4-D was cross-linked to bovine serum albumin (BSA, carrier) by 1-ethyl-3-(3-dimethyl- aminopropyl) carbodiimide hydrochloride (EDC). The conjugation reaction was found to be more effective at 4 degrees C and incubated for 18 hour. 2,4-D was dissolved in 0.05 mol/L phosphate buffer between 10.0 - 12.0 mg/mL concentration, pH was adjusted to be 5.4 - 6.1. When the weight of added EDC was below 12 mg, the more EDC was added the higher substitution degree complete immunogen was synthesized. Complete immunogen of various substitution degree (2,4-D: protein) were applied to immunize balb/c mice. The conjugates of 2,4-D and poly-L-lysine was applied as coating antigen. It was experimentally found that complete immunogen of substitution degree 12 and 18 are more immunogenic than that of substitution degree 6 and 25. None-specific adsorption between antiserum that was produced by complete immunogen of substitution degree 18 and coating antigen was very weak, and the antiserum contained more 2,4-D specific antibody. It could be used as the immunogen for the preparation monoclonal antibody.
%K 2
%K 4-Dichlorophenoxyacetic acid
%K complete immunogen
%K antiserum
%K immunogenic<br>2
%K 4-二氯苯氧乙酸
%K 完全抗原
%K 抗血清
%K 免疫原性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=287A401169A41E32&yid=37904DC365DD7266&vid=DB817633AA4F79B9&iid=CA4FD0336C81A37A&sid=A020552C37306588&eid=2DBBF45CC176713E&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=16