%0 Journal Article
%T The Application of the Gene-recombinated Cell in the Detection of PCBs Compounds in Environmental Samples
基因重组细胞在环境样品多氯联苯检测中的应用
%A XU Ting
%A KONG Fan-xiang
%A SUN Cheng
%A TAN Jian-kang
%A HAN Xiao-bo
%A YAN Rong
%A
徐挺
%A 孔繁翔
%A 孙成
%A 谈健康
%A 韩小波
%A 阎荣
%J 环境科学
%D 2004
%I
%X To develop novel and rapid bioassay systems for detection of PCBs in environmental and biological samples, two lines of gene-recombinated cells containing reporter gene, green fluorescent protein (GFP) and luciferase (Luc), were be chosen to detect the PCBs in the water, sediment and biological samples from environment. The result showed that the correlation between RFU of GFP and Luc activity and PCBs standard sample concentration was fine, r was 0.99188 and 0.98239 respectively. Compared with the instrument analysis with GC-ECD, the correlation between RFU of GFP and Luc activity and PCBs concentration in environmental and biological extracts was also fine. The method offered a new way of screening and semi-quantitative bioassay for PCBs compounds in environmental and biological samples.
%K gene-recombinated cell
%K PCBs compounds
%K environmental sample
基因重组细胞
%K 多氯联苯
%K 环境样品
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=CC63973B85118736&yid=D0E58B75BFD8E51C&vid=C5154311167311FE&iid=CA4FD0336C81A37A&sid=94E7F66E6C42FA23&eid=B6DA1AC076E37400&journal_id=0250-3301&journal_name=环境科学&referenced_num=9&reference_num=13