%0 Journal Article
%T Flow Injection Biosensor Based on the Immobilized AChE<br>基于固定化AChE的流动注射型酶传感器研究
%A ZHU Xiao-shan
%A MENG Fan-ping
%A ZHU Lin
%A HE Dong-hai
%A <br>朱小山
%A 孟范平
%A 朱琳
%A 何东海
%J 环境科学
%D 2006
%I 
%X A biosensor based on the flow injection system was constructed with the immobilized AChE from Scomberomorus niphonius(Curier) as identification element and a pH electrode as transducer.When phosphate buffer was used as carrier liquid,a good reproducibility(RSD=1.427%,n=10) of the biosensor response was obtained after the substrate was injected repetitively.After an incubation time of 20min,the calibration graph to methyl-parathion is linear(r=0.998?6)when its concentration ranges from 4.29×10-10 mol·L-1 to 4.29×10-8 mol·L-1,and the detection limit is 1.3×10-10 mol·L-1.However,the sensitivity of this biosensor to methyl-parathion when using clean seawater as carrier liquid is not as good as that using phosphate buffer.But after preoxidation of methyl-parathion with NaClO as oxidant,the detection limit of the biosensor to methyl-parathion in seawater can be improve to 2.16×10-7 mol·L-1.
%K immobilization
%K acetylcholinesterase(AChE)
%K biosensor
%K flow injection system
%K organophosphorus pesticides(OPs)<br>固定化
%K 乙酰胆碱酯酶
%K 生物传感器
%K 流动注射系统
%K 有机磷农药
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=2E65B174B2A20D72&yid=37904DC365DD7266&vid=DB817633AA4F79B9&iid=9CF7A0430CBB2DFD&sid=3381B1AF30824ACC&eid=86D7B9EF2FFFB112&journal_id=0250-3301&journal_name=环境科学&referenced_num=5&reference_num=24