%0 Journal Article
%T Preparation of Polyclonal Antibody Against Microcystin-LR
微囊藻毒素-LR多克隆抗体的制备
%A SHENG Jian-wu
%A HE Miao
%A HI Han-chang
%A QIAN Yi
%A
盛建武
%A 何苗
%A 施汉昌
%A 钱易
%J 环境科学
%D 2006
%I
%X Good quality polyclonal antibody against Microcystin-LR (MC-LR) was obtained from the New Zealand rabbit immunized with the homemade complete antigen BSA-MC-LR. Indirect ELISA shows the titer of this antibody is more than 1.5 x 10(5); Indirect competitive ELISA immobilized the coating antigen OVA-MC-LR was adapted to detect MCs, the calibration curve shows that the detection lower limit is 10 ng/L, and the range of quantitative detection was between 30 ng/L to 3 microg/L, with a good linearity, which will well potentially suit for sensitive analysis for MC-LR in drinking as well as surface water samples.
%K Microcystin-LR
%K polyclonal antibody
%K indirect competitive ELISA
微囊藻毒素-LR
%K 多克隆抗体
%K 间接竞争ELISA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=C5E98982041A62A6&yid=37904DC365DD7266&vid=DB817633AA4F79B9&iid=E158A972A605785F&sid=839A12D3ACF8C715&eid=CFBDB06850C21CC6&journal_id=0250-3301&journal_name=环境科学&referenced_num=2&reference_num=19