%0 Journal Article
%T Elertrofusion of Building Coupling System for Degrading Anthracene and Fusants'' Choices<br>选育蒽降解微生物耦合系统菌株的电融合及融合子的筛选
%A ZHANG Jia-yao
%A JI Miao
%A FANG Cheng-xiang
%A CHEN Fei-li
%A CHEN Ling
%A DENG Huan-huan
%A <br>张甲耀
%A 季淼
%A 方呈祥
%A 陈菲莉
%A 陈玲
%A 邓欢欢
%J 环境科学
%D 2004
%I 
%X Some researches have been carried out for the electro fusion of building coupling system for degrading anthracene and the choices of fusants. During the experiments, total rate of fusion stable in alternation current frequency 500 kHz, impulse intensity 300 V/cm, impulse width 5 micros, amount of impulse 2 and alternation current intensity 20-40 V/cm were found. The resistant plate with erythromycin and ampicillin can screen the fusant of autochthonous bacterium and surfactant-producing bacterium. The resistant plate with anthracene and karnamicin can screen the fusant of autochthonous bacterium and efficient anthracene-degradation bacterium. The new fusants were found to have stable fluorescent characteristics.
%K fusant
%K coupling system
%K electro fusion
%K resistant
%K efficient degradation bacterium
%K microbe<br>融合子
%K 耦合系统
%K 电融合技术
%K 抗性
%K 高效降解菌
%K 微生物
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=98D4F382B5BE6C57&yid=D0E58B75BFD8E51C&vid=C5154311167311FE&iid=CA4FD0336C81A37A&sid=2A3781E88AB1776F&eid=286FB2D22CF8D013&journal_id=0250-3301&journal_name=环境科学&referenced_num=3&reference_num=5