%0 Journal Article
%T Detection of Microcystin-LR in Waters Using Indirect Competitive ELISA<br>水体中微囊藻毒素-LR的间接竞争ELISA检测
%A SHENG Jian-wu
%A HE Miao
%A YU Shao-qing
%A SHI Han-chan
%A QIAN Yi
%A <br>盛建武
%A 何苗
%A 余少青
%A 施汉昌
%A 钱易
%J 环境科学
%D 2006
%I 
%X Indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established to detect microcystin-LR in waters, with the concentration of the complete antigen was 5microg/mL, the dilution of the monoclonal antibody was 1:3,000, the dilution of the enzyme tracer (goat anti-rabbit IgG-peroxidase) was 1:3,000, the concentration range of microcystin-LR was between 0.001 approximately 30microg/L, and using o-phenylenediamine as substrate. The assay showed a high relativity of more than 99% with high performance liquid chromatography, a mean relative standard deviation less than 10% , a detection limitation under 0.01microg/L and quantitative detection range was 0.01 approximately 3microg/L, high specificity for 4-arginine] microcystin, and it could still perform well under the influence from the samples.
%K microcystin-LR
%K monoclonal antibody
%K indirect competitive ELISA
%K detection<br>微囊藻毒素-LR
%K 单克隆抗体
%K 间接竞争ELISA
%K 检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=D11A05DFCE984977&yid=37904DC365DD7266&vid=DB817633AA4F79B9&iid=B31275AF3241DB2D&sid=811F346FF129CCCC&eid=75AB4EF959CC4ED9&journal_id=0250-3301&journal_name=环境科学&referenced_num=2&reference_num=20