%0 Journal Article
%T Effects of bistheonellide A, an Actin-polymerization Inhibitor, on Chinese Hamster V79 Cells and on IL-8 Production in PMA-stimulated HL-60 Cells
%A Taiko Oda
%A Jinzhong Xu
%A Ayako Fujita
%A Masataka Mochizuki
%A Michio Namikoshi
%J Marine Drugs
%D 2006
%I MDPI AG
%R 10.3390/md401022
%X Bioassay-guided isolation from the ethanol extract of a marine sponge Theonella sp. collected in Palau yielded bistheonellide A, which strongly inhibited the colony formation of Chinese hamster V79 cells (EC50 = 6.8 nM). Bistheonellide A is an actinpolymerization inhibitor and was suggested to control cytokine production. Therefore, we attempted to detect an effect of this compound on IL-8 production in PMA-stimulated HL- 60 cells. Interestingly, bistheonellide A did not modulate the production of IL-8 under cytotoxic concentrations as determined by LDH analysis. Although the correlation between the inhibition of microtubule assembly and the stimulation of IL-8 production has been observed for several compounds, the polymerization of actin was not related to an IL-8 production in the case of bistheonellide A. It will be suggested that the actin polymerization is not involved in the IL-8 production system.
%K Bistheonellide A
%K actin-polymerization inhibitor
%K IL-8
%K HL-60 cell
%K V79 cell
%U http://www.mdpi.com/1660-3397/4/1/22