%0 Journal Article
%T THE GH GENE DIVERSITY AMONG THREE SINIPERCA FISH SPECIES<br>三种鳜鱼(Siniperca)生长激素基因内含子多态性的比较研究
%A LIU Feng
%A LU Shuang-Qing
%A LIU Zhen
%A XIE Xin-Min
%A TANG Jian-Zhou
%A KUANG Gang-Qiao
%A <br>刘 峰
%A 鲁双庆
%A 刘 臻
%A 谢新民
%A 唐建洲
%A 匡刚桥
%J 海洋与湖沼
%D 2009
%I 
%X Siniperca chuatsi, S. kneri, S. schezeri are three major commercial fish species in genus Siniperca; they have close relationships and similar life habits, but significantly different growth. To understand the reason, the diversity of GH genes of three members is studied using PCR amplification, electrophoresis, and sequencing. Sequence diversities were observed in Intron 1, forepart of Intron 2, and microsatellite sites in Intron 2 and Intron 3. Twenty-five length types have been observed in all regions, among them 4 types are shared by S. chuatsi and S. kneri, and 3 types shared by three fishes; others were species-specific. All the length types in different regions formed 14 haplotypes, being species-specific. The sequence diversities are mainly due to repeat sequences and inserts or deletions of DNA fragments. There were two diversity loci close to the 3'-acceptor splice site of Intron 1 and the 5'-donor splice site of Intron 3 respectively. Phylogenetic tree was constructed based on diversity detections. S. chuatsi and S. kneri cluster into one clade before join S. schezeri. All these findings have laid a foundation for further study on the relationship between gene diversity and trait difference, as well as for further utilization of the diversity of GH gene in molecular breeding and germplasm conservation of the Siniperca fishes.
%K Mandarinfish
%K GH gene
%K Length polymorphism
%K Haplotype<br>鳜
%K 生长激素基因
%K 长度多态性
%K 单倍型
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=10F18692CD69EACA2DF7FC109993D35C&aid=49C95EEC0080C6E993EEFE4FB217F1F2&yid=DE12191FBD62783C&vid=1371F55DA51B6E64&iid=E158A972A605785F&sid=47F7649551A37CFC&eid=A94616C4391AA5EF&journal_id=0029-814X&journal_name=海洋与湖沼&referenced_num=1&reference_num=27