%0 Journal Article
%T CLONING AND PROKARYOTIC EXPRESSION OF GROWTH HORMONE GENE IN SINIPERCA CHUATSI<br>鳜(Siniperca chuatsi)生长激素基因克隆和原核表达
%A LIU Zhen
%A LUO Xiao-Hua
%A LU Shuang-Qing
%A XIE Di-Zhi
%A FANG Zhi-Jia
%A XIAO Ke-Yu
%A <br>刘臻
%A 罗小华
%A 鲁双庆
%A 谢帝芝
%A 房志家
%A 肖克宇
%J 海洋与湖沼
%D 2010
%I 
%X GH cDNA was amplified and cloned from total RNA isolated from pituitary gland of Siniperca chuatsi by RT-PCR. Sequence analysis showed an open reading frame (ORF) of S. chuatsi growth hormone gene in size of 615bp, encoding 204aa of 23kDa and pI at 7.07, comprising 10.78% acidic, 12.74% basic, 44.12% hydrophobic, and 32.35% hydrophilic. The cDNA of mature growth hormone was directionally cloned in pET-32a(+), and then the recombinant expression vector was transformed into E. coli BL21 (DE3 plys). After induction by IPTG, an expected 43kDa fusion protein was found by SDS PAGE analysis, accounting for 58% of the total protein. Western blot demonstrated that the recombinant fusion protein can be recognized by anti-6×His monoclonal antibody.
%K Siniperca chuatsi
%K Growth hormone
%K Gene clone
%K Prokaryotic expression<br>鳜
%K 生长激素
%K 基因克隆
%K 原核表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=10F18692CD69EACA2DF7FC109993D35C&aid=80142B87B5E2385CE1711370518F903C&yid=140ECF96957D60B2&vid=2001E0D53B7B80EC&iid=38B194292C032A66&sid=683005D16807E4FE&eid=0918129209B14F3E&journal_id=0029-814X&journal_name=海洋与湖沼&referenced_num=0&reference_num=19