%0 Journal Article %T CLONING AND EXPRESSION ANALYSIS OF THE REGULATORY SUBUNIT B GENE OF PP2A IN THE MUD CRAB SCYLLA PARAMAMOSAIN
拟穴青蟹(Scylla paramamosain)PP2A调节亚基B的基因克隆与表达分析 %A LIU Xue-Liang %A JIN Zhu-Xing %A HUANG Hui-Yang %A YE Hai-Hui %A LI Shao-Jing %A
刘学良 %A 金朱兴 %A 黄辉洋 %A 叶海辉 %A 李少菁 %J 海洋与湖沼 %D 2012 %I %X In this paper, the regulatory subunit B geneof PP2A (PP2A-B) was isolated from the mud crab, Scylla paramamosain using RT-PCR and RACE methods. The obtained full-length cDNA of PP2A-B was 2040bp with an open reading frame of 1332bp encoding a putative peptide of 443 amino acid. By alignment, the amino acid sequence of S. paramamosain PP2A-B showed high homology with those of some other animals. It suggested PP2A-B was highly conservative. Real-time PCR showed that the PP2A-B gene was expressed in various tissues, and highly expressed in brain, ovary and gill. The PP2A-B mRNA expression during ovarian development indicated that the expression of PP2A-B was obviously high at undeveloped stage and decreased gradually from stage I (undeveloped stage) to stage V (ripe stage). We hypothesized that PP2A-B may exist in the form of PP2A holoenzyme in ovary and play a suppressive role on the ovarian development of the mud crab. %K PP2A-B %K Scylla paramamosain %K Gene cloning %K Real-time RT-PCR %K Ovarian development
PP2A-B %K 拟穴青蟹 %K 基因克隆 %K 荧光定量PCR %K 卵巢发育 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=10F18692CD69EACA2DF7FC109993D35C&aid=4D6BB6A422263A45E79CD2A76B204545&yid=99E9153A83D4CB11&vid=BE33CC7147FEFCA4&iid=94C357A881DFC066&sid=796A97DD793AE4A8&eid=AB8B0EE7E1A96CB2&journal_id=0029-814X&journal_name=海洋与湖沼&referenced_num=0&reference_num=0