%0 Journal Article
%T EXPRESSION AND ACTIVITY ANALYSIS OF THIOREDOXIN (Trx) FROM MARINE COCCOLITHOPHORID EMILIANIA HUXLEYI VIRUS IN PICHIA PASTORIS<br>海洋球石藻病毒（Coccolithovirus）硫氧还蛋白（Trx）在毕赤酵母（Pichia pastoris）中的表达及其活性分析
%A CAI Yi-Qin
%A ZHANG Zhi-Lan
%A LUO Bang-Bin
%A LIU Jing-Wen
%A <br>蔡艺钦
%A 张稚兰
%A 罗邦彬
%A 刘静雯
%J 海洋与湖沼
%D 2012
%I 
%X Thioredoxin (Trx), a small molecule ubiquitous multifunctional acidic protein, was found to reduce disulfide bonds of protein (convert SOS to 2 SH) and thereby mitigate the allergenicity of food. Emiliania huxleyi virus (EhV) is the first virus containing Trx gene reported. Here the gene of EhV-Trx was amplified from the recombinant plasid of pBS-EhV99B1-Trx by PCR. Ehv-Trx gene was inserted yeast expression vector pPIC9K to construct recombinant expression plasmid pPIC9K-EhV99B1-Trx. After sequencing, the recombinant expression plasmid was transformed into Pichia pastoris GS115 by electroporation method. The recombinant protein EhV99B1-Trx reduction activity was detected. The results showed that: the open-reading frame (ORF) of EhV99B1-Trx encoded a protein of 197 amino acids; the recombinant EhV99B1-Trx was successfully induced expression in P. pastoris GS115 and the target protein molecular mass was about 27.8kDa; as found for other proteins with intramolecular disulfide bonds, insulin were reduced specifically by the recombinant EhV99B1-Trx and the treated product was relatively stable, which indicated that the EhV99B1-Trx, as a new kind of thioredoxin, do have the potential in food safety areas.
%K Emiliania huxleyi virus (EhV)
%K Thioredoxin (Trx)
%K Pichia pastoris
%K Secreted expression
%K Activity analysis<br>海洋球石藻病毒(EhV)
%K 硫氧还蛋白
%K 毕赤酵母
%K 分泌表达
%K 活性分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=10F18692CD69EACA2DF7FC109993D35C&aid=4D6BB6A422263A45999CE8C245C2F08B&yid=99E9153A83D4CB11&vid=BE33CC7147FEFCA4&iid=94C357A881DFC066&sid=FE2777263ADBA5FE&eid=A02B0E6E62BE4F0C&journal_id=0029-814X&journal_name=海洋与湖沼&referenced_num=0&reference_num=0