%0 Journal Article
%T RECOMBINATION AND EXPRESSION OF PATERNAL GENE OF ISOZYMES IN GYNOGENETIC OLIVE FLOUNDER PARALICHTHYS OLIVACEUS<br>雌核发育牙鲆同工酶基因的重组及父方基因的表达
%A XU Cheng
%A WANG Ke-Ling
%A XU Yong-Li
%A ZHANG Pei-Jun
%A <br>徐 成
%A 王可玲
%A 徐永立
%A 张培军
%J 海洋与湖沼
%D 2002
%I 
%X Gynogenetic induction of olive flounder Paralichthys olivaceus was carried out by cold shock after activation of the eggs with sperm which had been inactivated with UV irradiation. Biochemical samples of 57 gynogenetic flounder (in a family) were collected in May, 1999 8 isozymes, including LDH, IDHP, PGM, ACP, ADH, SDH, GDH, CAT, were analyzed by starch gel electrophoresis. These isozymes were polymorphic in wild population. Two loci, Ldh C and Cat had recombination in gynogenetic population. The recombination rates between isozyme locus and centromere were 52 6% and 29 8%, respectively. Paternal gene of Idhp 1 was found expressing at a low level in gynogenetic progenies. Mean proportion of polymorphic loci and the average heterozygosities of gynogenetic population were 7 0% and 0 035, respectively, much lower than that of wild population. The result suggests that gynogenesis involving retention of the second polar body is not a good method for making pure lines, and gynogenesis involving suppression of the first cell division may be feasible.
%K Paralichthys olivaceus
%K Gynogenesis
%K Isozyme
%K Recombination
%K Expression of paternal gene<br>牙Ping
%K 雌核发育
%K 同工酶
%K 基因重组
%K 父方基因表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=10F18692CD69EACA2DF7FC109993D35C&aid=D93A101C6BDC6A23&yid=C3ACC247184A22C1&vid=27746BCEEE58E9DC&iid=CA4FD0336C81A37A&sid=95D537AC89B28832&eid=5D71B28100102720&journal_id=0029-814X&journal_name=海洋与湖沼&referenced_num=10&reference_num=15