%0 Journal Article
%T CLONING, TISSUE EXPRESSION AND SINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS OF CULTER ALBURNUS TMEM-57<br>翘嘴鲌(Culter alburnus)TMEM-57基因的克隆、组织表达和单核苷酸多态性分析
%A QI Peng-Zh
%A GUO Bao-Ying
%A XIE Cong-Xin
%A WU Chang-Wen
%A LU Shi-Min
%A DUAN You-Jian
%A <br>祁鹏志
%A 郭宝英
%A 谢从新
%A 吴常文
%A 陆诗敏
%A 段友健
%J 海洋与湖沼
%D 2012
%I 
%X 采用RT-PCR和快速扩增cDNA末端(rapid amplification of cDNA ends, RACE)技术首次克隆了翘嘴鲌 TMEM-57蛋白基因的cDNA全序列, 该序列全长为2822bp, 其中5′-UTR区93bp, 3′-UTR区731bp, 开放阅读框(open reading frame, ORF)为1998bp, 编码665个氨基酸。NJ法系统进化分析将同科或属中TMEM-57基因分成一个分支, 表现出种属间的高度保守性。利用荧光定量PCR(qPCR)技术检测了不同组织的表达量, 发现在卵巢中表达量最高, 其次为心脏和脑。用直接测序法对TMEM-57基因部分区段单核苷酸多态性(SNP)进行了检测, 寻找到11个新的SNP位点。
%K Culter alburnus
%K TMEM-57
%K Real-time fluorescence quantitative PCR
%K Single nucleotide polymorphism<br>翘嘴鲌
%K TMEM-57
%K 荧光定量PCR
%K 单核苷酸多态性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=10F18692CD69EACA2DF7FC109993D35C&aid=74204B17C4981C5A273247416CCA3CC1&yid=99E9153A83D4CB11&iid=B31275AF3241DB2D&sid=D7FC8AF637611368&eid=B4942BBE94415B36&journal_id=0029-814X&journal_name=海洋与湖沼&referenced_num=0&reference_num=0