%0 Journal Article
%T SCREENING AND IDENTIFICATION OF VIBRIO-ANTAGONISTIC BACTERIA FROM THE GASTROINTESTINAL TRACT OF PSEUDOSCIAENA CROCEA<br>来自大黄鱼(Pseudosciaena crocea)肠道的弧菌拮抗菌的筛选与鉴定
%A WANG Juan
%A FENG Yong-Hui
%A CAI Li-Sheng
%A WANG Jian
%A QU Xian-Cheng
%A ZHANG Qing-Hua
%A <br>王娟
%A 封永辉
%A 蔡立胜
%A 王建
%A 曲宪成
%A 张庆华
%J 海洋与湖沼
%D 2010
%I 
%X One hundred and fifteen strains of bacteria were isolated from the gastrointestinal tract of cultured, healthy Pseudosciaena crocea and examined for their antagonistic activities against pathogenic Vibrio parahaemolyticus (Vp-1.2164) and Vibrio alginolyticus (Va-Y) by the dot-inoculating method. Three strains of bacteria (X1402-1, X1108 and X3914) inhibited the growth of the pathogenic vibrios. Strain X1402-1 showed the strongest antagonistic activity, with the diameters of inhibition zones of Vp-1.2164 and Va-Y being 28.5mm and 8.7mm, respectively. The diameters of inhibition zones of Vp-1.2164 and Va-Y were 12.0 and 9.0mm, respectively, for strain of X1108, and 0 and 7.2mm, respectively for strain X3914. In addition, their antibacterial spectra were studied. Strain X1402-1 showed the widest antibacterial spectra, inhibiting the growth of 16 out of the 21 strains of bacteria tested. Furthermore, identification of X1402-1, X1108 and X3914 was performed by two methods. With the VITEK-32 Bacteria Identification System (Biomerieux Company), X1402-1, X1108 and X3914 were recognized as Pseudomonas aeruginosa, Pseudomonas putida, and Bacillus licheniformis, respectively; their similarities were 99%. Complementally, the results of 16S rRNA gene sequence analysis showed that nucleotide homology of these three antagonistic strains to Pseudomonas pseudoalcaligenes (EU419918), Pseudomonas putida (FJ440105), and Bacillus licheniformis (DQ988136) were 100.0%, 97.5% and 99.0%, respectively. In summary, strains of X1402-1, X1108 and X3914 were concluded to be Pseudomonas pseudoalcaligenes, Pseudomonas putida and Bacillus licheniformis, respectively.
%K Dot-inoculating method
%K Antagonism
%K Antibacterial spectra
%K Screening
%K Bacteria identification<br>点种法
%K 拮抗作用
%K 抗菌谱
%K 筛选
%K 细菌鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=10F18692CD69EACA2DF7FC109993D35C&aid=24A8EF6AEA0F8D4554265A9794A1E10C&yid=140ECF96957D60B2&vid=2001E0D53B7B80EC&iid=94C357A881DFC066&sid=E008F9AD6D4B96EF&eid=A586B761C9AA2FAA&journal_id=0029-814X&journal_name=海洋与湖沼&referenced_num=0&reference_num=0