%0 Journal Article
%T STUDY ON THE DEVELOPMENT OF SNP MARKERS IN SCOPHTHALMUS MAXIMUS USING HIGH-RESOLUTION MELTING (HRM)<br>应用高分辨率熔解曲线(HRM)开发大菱鲆(Scophthalmus maximus)SNP标记的研究
%A LIU Qing-Ming
%A LI Meng
%A MA Ai-Jun
%A WANG Xin-An
%A HUANG Zhi-Hui
%A GUO Jian-Li
%A <br>刘庆明
%A 李 猛
%A 马爱军
%A 王新安
%A 黄智慧
%A 郭建丽
%J 海洋与湖沼
%D 2012
%I 
%X The experiment mines Scophthalmus maximus 12428 EST sequence from NCBI database. Putative SNP were detected from assembled contigs using Vector NTI Advance 11. SNP genotyping was performed using high-resolution melting to different character populations. Primers and probes were designed using Primer5 and Oligo7, respectively. The results show that, More than 160 putative SNP were found from 522 contigs; 56 putative SNP were selected for primer design and for SNP marker development, 37 pairs of primer were successfully amplified, including 45 putative SNP site, the success rate is 66.1%; Probes which can hybrid with Putative SNP sites have 13, hybridization Rate is 28.9%; 8 sites of hybridization sites displayed polymorphisms in different character populations, accounting for 61.5% of the hybrid sites. There are 6 transition sites, namely the G-A and C-T are 3 of each. There are 2 transversion sites, namely the T-G and A-T are 1 of each
%K Single nucleotide polymorphism (SNP)
%K Scophthalmus maximus
%K High-resolution melting analysis
%K EST sequence<br>单核苷酸多态性标记(SNP)
%K 大菱鲆
%K 高分辨率熔解曲线分析
%K EST
%K 序列
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=10F18692CD69EACA2DF7FC109993D35C&aid=74204B17C4981C5A82DC7BB0D156EC37&yid=99E9153A83D4CB11&iid=B31275AF3241DB2D&sid=39B73ADA6F3DD05F&eid=D5BEB939E141E547&journal_id=0029-814X&journal_name=海洋与湖沼&referenced_num=0&reference_num=0