%0 Journal Article
%T IN VITRO EVALUATION OF INTERLEUKIN-10 GENE DELIVERY INTO DORSAL ROOT GANGLION CELLS MEDIATED BY PEI-g-MPEG<br>PEI-g-MPEG/白细胞介素-10基因传递系统转染背根神经节细胞的体外性能研究
%A PEI Yuanyuan
%A WANG Weiwei
%A LIU Xianguo
%A SHUAI Xintao
%A <br>裴媛媛
%A 王伟伟
%A 刘先国
%A 帅心涛
%J 高分子学报
%D 2010
%I 
%X The use of non-viral carrier,polyethyleneimine-g-methoxypoly(ethylene glycol) (PEI-g-MPEG) for interleukin-10 (IL-10) gene delivery into rat dorsal root ganglion cells (DRGs) was examined in vitro.Electrophoresis,ethidium bromide exclusion,SEM,size and zeta (ζ) potential measurements were performed to characterize the nanocomplex,PEI-g-MPEG/pDNA.In addition,the cytotoxicity,transfection efficiency and IL-10 protein expression level in comparison with lipofectamine as a delivery agent were determined.Results showed that plasmid DNA was completely complexed by PEI-g-MPEG at a nitrogen-to-phosphor (N/P) ratio of 5.The PEI-g-MPEG/pDNA complexes were well-distributed and spherical with smooth surface.As the proportion of PEI-g-MPEG increased,i.e.when N/P ratio increased from 1 to 30,the size of the PEI-g-MPEG/DNA complexes decreased from 229 nm to 130 nm,while the surface charge increased from -19 mV to 32 mV,which led to the increased cytotoxicity.Flow cytometry revealed that the highest transfection efficiency (24%) using PEI-g-MPEG as a delivery agent was obtained at N/P charge ratio of 15,which was higher than that of lipofectamine (17%) at the same N/P value.After transfection,rat IL-10 expression in DRGs primary culture assessed using ELISA was observed to localize in the cytoplasm at 48 h in concordance with the result obtained for EGFP expression.These results indicated that PEI-g-MPEG could be a potential candidate for gene delivery during the therapy of neuropathic pain.
%K PEI-g-MPEG
%K DRGs
%K IL-10
%K Neuropathic pain<br>PEI-g-MPEG
%K DRGs
%K IL-10
%K 神经病理性疼痛
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=6579068328FE643F&jid=15971983683C7643EE80F1CE621DEB60&aid=CD49411EF74D1C3E4A7B78A4756DB8E7&yid=140ECF96957D60B2&iid=CA4FD0336C81A37A&sid=9C65ADEB5990B252&eid=7AA74D31F1FF2DCE&journal_id=1000-3304&journal_name=高分子学报&referenced_num=0&reference_num=20