%0 Journal Article
%T Cloning and Effective Constitutive Expression of aspC Gene in Escherichia coli<br>组成型天冬氨酸转氨酶基因工程菌的构建与高效表达
%A GONG Chang-bin
%A XU Xian
%A LI Shuang
%A HE Bing-fang
%A <br>宫长斌
%A 徐娴
%A 李霜
%A 何冰芳
%J 过程工程学报
%D 2007
%I 
%X L-phenylalanine(L-Phe) can be prepared from phenylpyruvate(PPA) via an amination reaction mediated by aspartate aminotransferase(encoded by aspC) as the key enzyme.In this study,the aspC gene was cloned into plasmid pUC18,pSE 380 and pET-22b to construct three kinds of constitutive vectors,which were then transferred into 6 strains of Escherichia coli,respectively,to over-produce aminotransferase.From the transformants,the E.coli strain BL21(DE3) harboring plasmid pET/P-aspC showing the highest activity was scored and designated BL21(pET/P-aspC).With L-Asp and PPA(20 g/L) as substrates,the conversion rate reached 80.1% after eight-hour reaction,approaching the theoretical yield.This system had good industrial prospects because no inducer or coenzyme was needed.Furthermore,the results showed the potential of gene engineering on strain selections.
%K constitutive expression
%K aspC
%K aspartate aminotransferase(AspAT)
%K L-phenylalanine(L-Phe)
%K pyridoxal 5'-phosphate(PLP)<br>组成型表达
%K 天冬氨酸转氨酶基因
%K 天冬氨酸转氨酶
%K L-苯丙氨酸
%K 磷酸吡哆醛
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=DE8A0188B82CBFD2C24EDB5A0739DCAF&yid=A732AF04DDA03BB3&vid=DF92D298D3FF1E6E&iid=38B194292C032A66&sid=F6467E7C54ED952D&eid=C2053D4E59904B8A&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=2&reference_num=17