%0 Journal Article
%T Separation,Purification and Characterization of Three Endo-polygalacturonases from a Newly Isolated Penicillum oxalicum<br>Separation, Purification and Characterization of Three Endo-polygalacturonases from a Newly Isolated Penicillum oxalicum
%A ZHANG Chun-hui
%A LI Zu-ming
%A PENG Xia-wei
%A JIA Yue
%A ZHANG Hong-xun
%A BAI Zhi-hui
%A <br>张春晖
%A 李祖明
%A 彭霞薇
%A 贾月
%A 张洪勋
%A 白志辉
%J 过程工程学报
%D 2009
%I 
%X Three endo-polygalacturonases (endoPGs) from a newly isolated Penicillum oxalicum (CGMCC 0907) capable of utilizing waste biomass as growth substrate were separated and purified to homogeneity by ultra-filtration, affinity adsorption chromatography, CM-cellulose column chromatography, and Sephadex G-100 gel filtration chromatography with the overall yield of 64.5% from the crude enzyme. The specific activities and recovery rates of endoPG-1, endoPG-2 and endoPG-3 were 1120 U/mg and 21.6%, 1350 U/mg and 25.9%, and 1 560 U/mg and 17.0%, respectively. The three purified endoPGs had a close molecular weight to 41 kDa as estimated by SDS-PAGE. The optimum temperature and pH for the function of them were 65℃ and 5.0, 55℃ and 5.0, 50℃ and 5.5, respectively. Their pI and Km values were 5.9 and 0.78 mg/mL, 6.0 and 1.2 mg/mL, and 6.1 and 2.0 mg/mL, respectively.
%K separation
%K characterization
%K Penicillum oxalicum
%K endo-polygalacturonase<br>多聚半乳糖醛酸酶
%K 分离
%K 提取
%K 净化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=6941239DC56AFE31D3D3501C5D02701C&yid=DE12191FBD62783C&vid=9CF7A0430CBB2DFD&iid=0B39A22176CE99FB&sid=0C191C6ECF79047F&eid=D537C66B6404FE57&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=0&reference_num=34