%0 Journal Article
%T Study on Dilution and Ion Exchange Chromatographic Refolding of Recombinant Human Granulocyte Colony-stimulating Factor by Reversed Phase Chromatography<br>反相色谱法研究人粒细胞集落刺激因子的离子交换色谱复性和稀释复性
%A LUO Tian-le
%A DING Hong
%A LI Jing-jing
%A SU Zhi-guo
%A <br>罗天乐
%A 丁虹
%A 李京京
%A 苏志国
%J 过程工程学报
%D 2005
%I 
%X A novel way to refold rhG-CSF by combining ion exchange chromatography (IEC) and L-arginine was developed: denatured rhG-CSF from inclusion body was attached to a DEAE column and eluted by a gradient from 6 mol/L urea to 0.5 mol/L L-arginine. Refolding of rhG-CSF was also done by dilution and by passing the rhG-CSF inclusion body through the DEAE column perfused with 0.5 mol/L arginine without adsorption. RPC analysis and bioactivity assay showed that rhG-CSF refolded by IEC had different pattern from those of the rhG-CSFs refolded by dilution and flowing-through, representing fast but asynchronous kinetics, due to the adsorption of the DEAE medium to the rhG-CSF and the gradient of denaturant. It was also found that subsequent dilution and incubation of the IEC-refolded rhG-CSF furthered its refolding and enhanced its bioactivity up to 10 folds.
%K rhG-CSF
%K inclusion body
%K ion exchange chromatography
%K refolding
%K arginine
%K dilution
%K reversed phase chromatography<br>重组人粒细胞集落刺激因子
%K 精氨酸
%K 包含体
%K 复性
%K 反相色谱
%K 离子交换色谱
%K 稀释
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=006F537D7DB59D6D&yid=2DD7160C83D0ACED&vid=94C357A881DFC066&iid=0B39A22176CE99FB&sid=527AEE9F3446633A&eid=CEC789B3C68C3BB3&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=1&reference_num=8