%0 Journal Article
%T Preparation of Heparin-Agarose Gel 6FF and Its Application in the Purification of Antithrombin Ⅲ<br>肝素-琼脂糖凝胶6FF的制备及其在抗凝血酶III分离纯化中的应用
%A MU Cheng-hua
%A YAO Hong-juan
%A MA Guang-hui
%A LIAN Bin State Key Lab Biochem Eng
%A Inst Process Eng
%A Chinese Academy of Sciences
%A Beifing
%A China
%A Inst Chem BiotechnoL
%A Guizhou University
%A Guiyang
%A Guizhou
%A China
%A <br>穆成华
%A 姚红娟
%A 马光辉
%A 连宾
%J 过程工程学报
%D 2005
%I 
%X 以自制琼脂糖凝胶6FF为基质，肝素为配基，利用还原胺化方法制备了肝素-琼脂糖凝胶6FF介质．考察了肝素偶联反应的影响因素，并对其进行了优化，得到肝素配基的含量为2．7mg／mL用所制介质从人血浆中分离出抗凝血酶Ⅲ，从血浆开始计算的抗凝血酶Ⅲ活性回收率为31．76％，与商品Heparin-Sepharose CL-6B的分离效果相当。
%K 亲和层析
%K 肝素
%K 抗凝血酶Ⅲ
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=9CEAD3222848B3B7&yid=2DD7160C83D0ACED&vid=94C357A881DFC066&iid=94C357A881DFC066&sid=6313C162FF75889A&eid=811ACA5D3673A764&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=3&reference_num=14