%0 Journal Article
%T Research on Superparamagnetic Polyvinyl Acetate Microspheres for Affinity Separation of Nattokinase<br>超顺磁性聚醋酸乙烯酯微球亲和分离纳豆激酶
%A KONG Peng
%A XIE Yu-chun
%A WEI Xue-tuan
%A XU Lin
%A YANG Liang-rong
%A LI Peng-fei
%A ZHOU Hua-cong
%A LIU Hui-zhou
%A <br>孔鹏
%A 谢渝春
%A 魏雪团
%A 徐林
%A 杨良嵘
%A 李鹏飞
%A 周华从
%A 刘会洲
%J 过程工程学报
%D 2012
%I 
%X Micro-size magnetic polyvinyl acetate microspheres were prepared by a modification suspension polymerization,the magnetic beads were further modified with hydrolysis and aminization reactions,and magnetic affinity microspheres suitable for nattokinase purification were obtained by covalent immobilization of p-aminobenzamidine to the amino-modified magnetic beads by the glutaraldehyde method.The properties of magnetic beads were examined by SEM,XRD,FT-IR and VSM.The results showed that these magnetic beads were typically superparamagnetic and the specific saturation magnetization of ligand-attached magnetic beads was 32.4 emu/g.It took only 40 min to reach adsorption equilibrium and less than 15 min to achieve desorption equilibrium.The purification factor and the recovery rate of enzyme activity were 10.3 and 73.4%,respectively.The purified nattokinase gave a single sharp band on SDS-PAGE.
%K nattokinase
%K affinity separation
%K purification
%K adsorption
%K kinetics<br>纳豆激酶
%K 磁性PVA微球
%K 亲和分离
%K 吸附
%K 动力学
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=28538CA98C300078DC6B643B7F63F9F9&yid=99E9153A83D4CB11&vid=59906B3B2830C2C5&iid=CA4FD0336C81A37A&sid=03F1579EF92A5A32&eid=480C51B1F0CE0AB6&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=0&reference_num=0