%0 Journal Article
%T Preparation of(R)-1-Phenyl-1,2-ethanediol by Biotransformation with a Coupled System of(R)-Specific Carbonyl Reductase and Formate Dehydrogenase<br>羰基还原酶与甲酸脱氢酶偶联催化制备(R)-苯基乙二醇
%A ZHANG Rong-zhen
%A XU Yan
%A GENG Ya-wei
%A WANG Shan-shan
%A SUN Ying
%A <br>张荣珍
%A 徐岩
%A 耿亚维
%A 王珊珊
%A 孙莹
%J 过程工程学报
%D 2009
%I 
%X The effects of reaction temperature,pH value,initial substrate concentration,reaction duration,cell concentration and forms on biotransformation reaction for preparation of(R)-1-phenyl-1,2-ethanediol were studied with 2-hydroxyacetophenone and sodium formate as substrates and the whole cells of E.coli Rosetta/pETDuet-rcr-fdh as catalyst.The results showed that when the concentration of 2-hydroxyacetophenone was 6 g/L,(R)-1-phenyl-1,2-ethanediol was produced with high optical purity of 98.37% enantiomeric ex...
%K 重组大肠杆菌
%K 生物催化
%K (R)-苯基乙二醇
%K 光学纯度
%K 得率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=F57EAFA33284E7F4E39B8BC5BD26EEEF&yid=DE12191FBD62783C&vid=9CF7A0430CBB2DFD&iid=E158A972A605785F&sid=0018E43E61963A72&eid=8C8D39B86A1EED4F&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=0&reference_num=18