%0 Journal Article
%T Amino Acid Metabolism of Vero Cells in Batch Culture<br>Vero细胞批式培养中的氨基酸代谢
%A GAO Hong-liang
%A CONG Wei
%A OUYANG Fan
%A <br>高红亮
%A 丛威
%A 欧阳藩
%J 过程工程学报
%D 2001
%I 
%X Amino acids are very important nutrients in mammalian cell cultivation. Amino acids metabolism varies greatly from one cell line to another, and specific culture media were therefore designed to cover the needs of individual lines. The paper investigated the amino acid metabolism of Vero cells in batch culture by HPLC. The results showed that 12 amino acids (Gln, Arg, His, Leu, Thr, Ile, Lys, Ser, Tyr, Phe, Val and Met) were consumed, and 4 amino acids (Glu, Asp, Ala and Pro) were produced during the growth of Vero cells. 6 amino acids ( Leu, Thr, Ile, Lys, Phe and Tyr) were consumed quickly only in the exponential phase of growth and consumed very slowly in the stationary phase of growth.
%K Vero cell
%K amino acid
%K metabolism<br>Vero细胞
%K 氨基酸
%K 代谢
%K 批式培养
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=825F3DC394AC47BE&yid=14E7EF987E4155E6&vid=CA4FD0336C81A37A&iid=0B39A22176CE99FB&sid=5BC9492E1D772407&eid=E114CF9BB47B65BE&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=1&reference_num=12