%0 Journal Article
%T Isolation and Cultivation of Acidithiobacillus ferrooxidans and Its Effects on Bioleaching of Phosphate Ore<br>氧化亚铁硫杆菌的分离培养及其浸磷效果
%A GONG Wen-qi
%A CHEN Wei
%A ZHANG Xiao-zheng
%A BIAN Xun
%A LIU Yan-ju
%A LIU Jun
%A HUANG Yong-bing
%A YANG Hong-gang
%A <br>龚文琪
%A 陈伟
%A 张晓峥
%A 边勋
%A 刘艳菊
%A 刘俊
%A 黄永炳
%A 杨红刚
%J 过程工程学报
%D 2007
%I 
%X A strain of Acidithiobacillus ferrooxidans that can be used effectively in the leaching of low grade phosphate ore was isolated from acid mine drainage from a coal mine in Anhui Province in China.Its characteristics of morphology and growth were studied.Through the investigation of the effects of different solid culture medium components on the isolation and purification of Acidthiobacillus ferrooxidans,the optimum isolation conditions were determined as:the concentration of Fe2+ in 9K solid culture medium was 4.5 g/L.A double-layer plate,with heterotrophic microbe Rhodotorula sp.spreading onto the bottom layer and chemoautotrophic bacterium At.f spreading onto the upper layer,was used.The influential factors including culture media,energy source materials and the addition of surfactant on the leaching of phosphate ore with the cultivated At.f were studied.Through preliminary experiments,up to 48% of phosphate in the ore was leached out using the isolated At.f strain.
%K Acidithiobacillus ferrooxidans
%K isolation and purification
%K solid culture medium
%K phosphate ore dissolution
%K bacteria/ leaching
%K phosphate leaching rate<br>氧化亚铁硫杆菌
%K 分离纯化
%K 固体培养基
%K 磷矿分解
%K 细菌浸出
%K 浸磷率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=E0BD45C9110E10605E4DBBD48F29AE76&yid=A732AF04DDA03BB3&vid=DF92D298D3FF1E6E&iid=38B194292C032A66&sid=E04FC1B5BC47587B&eid=4964C30D71DF45FF&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=4&reference_num=13