%0 Journal Article
%T Optimizing Conditions for Bacteriocin Isolation and Purification in Temperature-induced Aqueous Two-phase Systems<br>温度诱导双水相分离纯化细菌素工艺条件的选取与优化
%A LI Chan
%A CAI Zhao-ling
%A CONG Wei
%A BAI Jing-hua
%A OUYANG Fan
%A <br>李孱，蔡昭铃
%A 丛威
%A 白景华
%A 欧阳藩
%J 过程工程学报
%D 2001
%I 
%X Bacteriocin nisin was isolated and purified in temperature-induced aqueous two-phase systems using the capacity of extracting hydrophobic proteins of TritonX-114. The concentrations of sodium chloride and TritonX-114which can form the aqueous two-phase systems were optimized by the response surface method. The full-factorial design was used to assess the main effects of factors and determine the path of steepest ascent to the optimal region of the aqueous two-phase system composition. The optimum concentrations of TritonX-114 and sodium chloride were then easily determined by using a central composite design and were found to be 3.27% of TritonX-114 and 0.85 mol/L sodium chloride. The recovery and purification factor were 92% and 9.05 respectively under the optimum conditions.
%K temperature-induced aqueous two-phase systems
%K extraction
%K bacteriocin
%K response surface methods
%K optimization<br>温度诱导双水相系统
%K 萃取
%K 细菌素
%K 响应面法
%K 优化
%K 食品添加剂
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=0857F0E3D6A0F892&yid=14E7EF987E4155E6&vid=CA4FD0336C81A37A&iid=E158A972A605785F&sid=216EFB25F7F834CC&eid=BFB86B6ED3A99B9D&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=0&reference_num=9