%0 Journal Article
%T Application of Experimental Design to Optimizing the Medium of Nuclease P1<br>实验设计法优化核酸酶P1的发酵培养基
%A XU Zheng-jun
%A XIAO Lin-ping
%A LU Hao
%A XIE Ning-chang
%A YING Han-jie
%A <br>徐正军，肖林平，吕浩，谢宁昌，应汉杰
%J 过程工程学报
%D 2003
%I 
%X Experimental design was employed to study the effects of several factors on the production of nuclease P1 by Penicillium citrinum M02, such as carbon source, nitrogen source, phosphor source and corn steep liquor which acted as complementary nitrogen source. The results of two experiments showed that corn steep liquor could remarkably promote the production of nuclease P1. In addition, one model that can describe the fermentation perfectly was gained from these experiments. When nuclease P1 was produced on the optimized medium calculated from this model, its yield was 648.3 U/ml that is comparable with 661 U/ml from the model. And it is improved by 70% compared with the 380 U/ml, which was gained on the original medium at the same time. The possible reason for corn steep liquor promoting nuclease P1 fermentation is that corn steep liquor contains more useful amino acids such as glycin, alanine, serine and so on.
%K Penicillium citrinum M02
%K nuclease P1
%K fermentation medium
%K corn steep liquor
%K experimental design
%K optimization<br>桔青霉M02
%K 核酸酶Pl
%K 发酵培养基
%K 玉米浆
%K 实验设计
%K 优化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=42E0581DCFB9E5F8&yid=D43C4A19B2EE3C0A&vid=38B194292C032A66&iid=94C357A881DFC066&sid=DDEED1BDDBFAA8A7&eid=B40AD8FE6FA88DE9&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=4&reference_num=15