%0 Journal Article
%T Effect of Submerged/Unsubmerged Period on Cell Culture of Saussurea medusa in a Novel Air-lift Bioreactor<br>新型气升式反应器中浸没/非浸没周期对水母雪莲细胞培养的影响
%A YUAN Xiao-fan
%A CHEN Shu-an
%A ZHAO Bing
%A WANG Yu-chun
%A LIU De-ming
%A <br>袁晓凡，陈书安，赵兵，王玉春，刘德明
%J 过程工程学报
%D 2003
%I 
%X The alternant submerged/unsubmerged cell culture of Saussurea medusa in a novel 1.8 L periodically submerged air-lift bioreactor was investigated. The bioreactor contains two layers of meshes. They provide support for cells in unsubmerged culture, but when being pulled slant the culture can be released by circulating medium from the meshes for submerged culture. The effects of the submerged/unsubmerged period on cell growth, biosynthesis of flavonoids and cell aggregate were studied. It was found that 5 min/4 h was the optimum submerged/unsubmerged period when the culture was carried out at air flow rate of 40 L/h and an inoculum (DW) of 2-3 g/L. After 20 d culture, the maximum biomass and production of flavonoids reached 9.6 g/L and 338 mg/L respectively, and the distribution of cell aggregates was the optimal as well.
%K air-lift bioreactor
%K submerged/unsubmerged period
%K Saussurea medusa
%K flavonoids<br>水母雪莲
%K 细胞培养
%K 气升式反应器
%K 浸没／非浸没周期
%K 黄酮
%K 生物反应器
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=D3E32F6885816EEE&yid=D43C4A19B2EE3C0A&vid=38B194292C032A66&iid=E158A972A605785F&sid=3E3EF0DB5E6F2DA9&eid=683005D16807E4FE&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=0&reference_num=12