%0 Journal Article
%T Dependence of The Resolution of a Fluorescent Confocal Microscopy on Fluorescent Spectrum<br>荧光谱线分布对共焦荧光显微镜分辨率的影响
%A Yang Chuping
%A Tan Moyan
%A Song Qing
%A XI Gang
%A <br>杨初平
%A 谭默言
%A 宋清
%A 习岗
%J 光子学报
%D 2005
%I 
%X The dependence of the resolution of a fluorescent confocal microscopy on fluorescent spectrum is studied, and the power transfer function and three-dimensional pulse spread function are derived. Assuming well-distributed fluorescent spectrum, calculated results show that the lateral resolution and the Axial resolution are worse while fluorescent spectrum widen and dispersion coefficient increased, compared with that by only using the central fluorescent wavelength. The detector responding to narrower fluorescent spectrum is good for the resolution.
%K Fluorescent confocal microscopy
%K Fluorescent spectrum
%K Resolution<br>共焦荧光显微镜
%K 荧光谱线分布
%K 分辨率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=47EA7CFDDEBB28E0&jid=9F6139E34DAA109F9C104697BF49FC39&aid=160E94F53A49DDF5&yid=2DD7160C83D0ACED&vid=339D79302DF62549&iid=B31275AF3241DB2D&sid=90EAFEE49150CFCD&eid=40541F483644ACC4&journal_id=1004-4213&journal_name=光子学报&referenced_num=0&reference_num=11