%0 Journal Article
%T Detection Limit of DNA Analyzer<br>DNA分析仪的灵敏度分析
%A WANG Jie
%A WANG Li-qiang
%A SHI Yan
%A ZHENG Hua
%A LU Zu-kang
%A <br>汪洁
%A 王立强
%A 石岩
%A 郑华
%A 陆祖康
%J 光子学报
%D 2008
%I 
%X The sensitivity of the DNA analyzer, which was designed by authors group to detect the samples of capillary electrophoresis (CE) with Laser induced fluorescence (LIF), was analyzed in theory and experiment. The detection sensitivity of fluorescence depends on both the fluorescent intensity entering the sensors (PMT or CCD) and the performance of sensors. Given an actual DNA analyzer, the calculated detection limit of TO(thiazole orange) fluorophore was 0.127 1~ 0.127 2 fluors per cubic micron. The experimental result of CE about double-stranded (ds) TO probes (the standard sample pGEM-3Zf(+)/Hae III Markers) is 0.145 4 fluors per cubic micron. The study shows that the approaches for improving detection limit are to make the good coating on inner walls of capillary, choose the suitable sieving matrix, enlarge laser power, increase numerical aperture of objective as much as possible and raise the detection lower limit of photomultiplier.
%K DNA analyzer
%K Sensitivity
%K Laser induced fluorescence
%K Capillary electrophoresis<br>DNA分析仪
%K 灵敏度
%K 激光诱导荧光
%K 毛细管电泳
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=47EA7CFDDEBB28E0&jid=9F6139E34DAA109F9C104697BF49FC39&aid=438ED256B6C5274EF239D40BE323C493&yid=67289AFF6305E306&vid=42425781F0B1C26E&iid=38B194292C032A66&sid=BA305A52E2EE9350&eid=DEBDB7F30FBA7F9B&journal_id=1004-4213&journal_name=光子学报&referenced_num=0&reference_num=12