%0 Journal Article
%T Limit Penetration Depth of Microscopic Imaging Caused by Focusing Light Into the Bulk Specimen Through Planar Interfaces Between Materials of Mismatched Refractive Index
界面折射率不一致对显微成像穿透深度的限制
%A (
%A
高万荣
%A 胡茂海
%A 杨晓春
%A 张仙玲
%A 谢仁龙
%A 沈为民
%J 光子学报
%D 2005
%I
%X Applications of confocal scanning microscopy and low coherence tomography in the biological and material sciences for optical-sectioning imaging require focusing light into the bulk specimen.However, the differences of refractive indices among biological tissues,the cover slip and immersion medium cause the strong spherical aberration and make the electromagnetic distribution in the focal region distorted.The spherical aberrations caused by focusing the light deeply into the specimen through multi-layer medium with different refractive index are analyzed. The results are used to calculate the limit penetration depth of microscopic imaging caused by focusing light into the bulk specimen through planar interfaces between materials of mismatched refractive index.The effect of spherical aberration on electromagnetic distribution in the focal region is also discussed.
%K Mismatched refractive
%K Microscopy
%K Penetration depth
折射率
%K 显微成像
%K 穿透深度
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=47EA7CFDDEBB28E0&jid=9F6139E34DAA109F9C104697BF49FC39&aid=F96657343D6FB50C&yid=2DD7160C83D0ACED&vid=339D79302DF62549&iid=B31275AF3241DB2D&sid=725C082EB6FD280F&eid=43AADF4B53A8BF6F&journal_id=1004-4213&journal_name=光子学报&referenced_num=0&reference_num=8