%0 Journal Article
%T High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435
%A Chia-Hung Kuo
%A Hsin-Hung Chen
%A Jiann-Hwa Chen
%A Yung-Chuan Liu
%A Chwen-Jen Shieh
%J International Journal of Molecular Sciences
%D 2012
%I MDPI AG
%R 10.3390/ijms130911694
%X Wax esters are long-chain esters that have been widely applied in premium lubricants, parting agents, antifoaming agents and cosmetics. In this study, the biocatalytic preparation of a specific wax ester, cetyl octanoate, is performed in n-hexane using two commercial immobilized lipases, i.e., Lipozyme £¿ RMIM ( Rhizomucor miehei) and Novozym £¿ 435 ( Candida antarctica). Response surface methodology (RSM) and 5-level-4-factor central composite rotatable design (CCRD) are employed to evaluate the effects of reaction time (1¨C5 h), reaction temperature (45¨C65 ¡ãC), substrate molar ratio (1¨C3:1), and enzyme amount (10%¨C50%) on the yield of cetyl octanoate. Using RSM to optimize the reaction, the maximum yields reached 94% and 98% using Lipozyme £¿ RMIM and Novozym £¿ 435, respectively. The optimum conditions for synthesis of cetyl octanoate by both lipases are established and compared. Novozym £¿ 435 proves to be a more efficient biocatalyst than Lipozyme £¿ RMIM.
%K optimization
%K wax esters
%K lipase
%K esterification
%K Rhizomucor miehei
%K Candida antarctica
%U http://www.mdpi.com/1422-0067/13/9/11694