%0 Journal Article
%T Quantification and Purification of Mangiferin from Chinese Mango (Mangifera indica L.) Cultivars and Its Protective Effect on Human Umbilical Vein Endothelial Cells under H2O2-induced Stress
%A Fenglei Luo
%A Qiang Lv
%A Yuqin Zhao
%A Guibing Hu
%A Guodi Huang
%A Jiukai Zhang
%A Chongde Sun
%A Xian Li
%A Kunsong Chen
%J International Journal of Molecular Sciences
%D 2012
%I MDPI AG
%R 10.3390/ijms130911260
%X Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango ( Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH £¿ free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H 2O 2-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H 2O 2 stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.
%K Mangifera indica
%K mangiferin
%K purification
%K antioxidant
%K HUVEC
%K protective effect
%U http://www.mdpi.com/1422-0067/13/9/11260