%0 Journal Article
%T Hepatoprotective Effects of Berberis vulgaris L. Extract/¦Â Cyclodextrin on Carbon Tetrachloride¨CInduced Acute Toxicity in Mice
%A Anca Hermenean
%A Cristina Popescu
%A Aurel Ardelean
%A Miruna Stan
%A Nicoleta Hadaruga
%A Ciprian-Valentin Mihali
%A Marieta Costache
%A Anca Dinischiotu
%J International Journal of Molecular Sciences
%D 2012
%I MDPI AG
%R 10.3390/ijms13079014
%X The present study investigated the capacity of formulated Berberis vulgaris extract/¦Â-cyclodextrin to protect liver against CCl 4-induced hepatotoxicity in mice. Formulated and non-formulated extracts were given orally (50 mg/kg/day) to mice for 7 days and were then intra-peritoneally injected with 1.0 mL/kg CCl 4 on the 8th day. After 24 h of CCl 4 administration, an increase in the levels of apartate-amino-transferase (AST), alanine-amino-transferase (ALT) and malondialdehyde (MDA) was found and a significant decrease in superoxide-dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-peroxidase (GPx) levels could be detected. This was accompanied by extended centrilobular necrosis, steatosis, fibrosis and an altered ultrastructure of hepatocytes. Pre-treatment with formulated or non-formulated extract suppressed the increase in ALT, AST and MDA levels and restored the level of antioxidant enzymes at normal values. Histopathological and electron-microscopic examination showed milder liver damage in both pre-treated groups and the protective effect was more pronounced after the formulated extract was administered. Internucleosomal DNA fragmentation induced by CCl 4 was reduced in the group which received non-formulated extract and absent in the group which received formulated extract. Taken together, our results suggest that Berberis vulgaris/¦Â-cyclodextrin treatment prevents hepatic injury induced by CCl 4 and can be considered for further nutraceutical studies.
%K Berberis vulgaris extract
%K ¦Â-cyclodextrin
%K hepatoprotection
%K oxidative stress
%U http://www.mdpi.com/1422-0067/13/7/9014