%0 Journal Article
%T Bio-Guided Isolation of the Cytotoxic Terpenoids from the Roots of Euphorbia kansui against Human Normal Cell Lines L-O2 and GES-1
%A Li Zhang
%A Lan Gao
%A Zhengjun Li
%A Xiaojing Yan
%A Yanjing Yang
%A Yuping Tang
%A Yudan Cao
%A Anwei Ding
%J International Journal of Molecular Sciences
%D 2012
%I MDPI AG
%R 10.3390/ijms130911247
%X The dried roots of Euphorbia kansui (kansui) have been used for centuries in China as a herbal medicine for edema, ascites, and asthma. The 95% ethanol extract showed a significant inhibition of cell proliferation against human normal cell lines L-O2 and GES-1. Bioassay-guided separation of the 95% ethanol extract from the roots of E. kansui led to the isolation of 12 diverse terpenoids whose structures were identified by 1H, 13C NMR spectroscopy and ESI-MS as kansuinine A ( 1), kansuinine B ( 2), kansuinine C ( 3), kansuiphorin C ( 4), 3- O-(2' E,4' Z-decadienoyl)-20- O-acetylingenol ( 5), 3- O-(2' E,4' E-decadienoyl)-20- O-acetylingenol ( 6), 3- O-(2' E,4' Z-decadienoyl)-20-deoxyingenol ( 7), 3- O-benzoyl-20-deoxyingenol ( 8), 5- O-benzoyl-20-deoxyingenol ( 9), kansenone ( 1 0), epi-kansenone ( 11), euphol ( 12). All these 12 terpernoids were evaluated in vitro for cytotoxicity on L-O2 and GES-1 cell lines. Most ingenane-type diterpenoids and 8-ene-7-one triterpenoids ( 5¨C 11) exhibited a relatively lower IC 50 value; therefore, these compounds had stronger cytotoxicity against human normal cell lines L-O2 and GES-1 with dose-dependent relationships. These results will be signi£¿cantly helpful to reveal the mechanism of toxicity of kansui and to effectively guide safer clinical application of this herb.
%K Euphorbia kansui
%K terpenoids
%K cytotoxicity
%K bio-guided separation
%K human normal liver cell lines L-O2
%K human normal gastric epithelial cell line GES-1
%U http://www.mdpi.com/1422-0067/13/9/11247