%0 Journal Article
%T Anti-TNF-汐 Activity of Portulaca oleracea in Vascular Endothelial Cells
%A An Sook Lee
%A Jin Sook Kim
%A Yun Jung Lee
%A Dae Gill Kang
%A Ho Sub Lee
%J International Journal of Molecular Sciences
%D 2012
%I MDPI AG
%R 10.3390/ijms13055628
%X Vascular inflammation plays a key role in the pathogenesis and progression of atherosclerosis, a main complication of diabetes. The present study investigated whether an aqueous extract of Portulaca oleracea (AP) prevents the TNF-汐-induced vascular inflammatory process in the human umbilical vein endothelial cell (HUVEC). The stimulation of TNF-汐 induced overexpression of adhesion molecules affects vascular cell adhesion molecule (VCAM)-1, intercellular adhesion molecule (ICAM)-1 and E-selectin for example. However, AP significantly suppressed TNF-汐-induced over-expression of these adhesion molecules in a dose-dependent manner. In addition, pretreatment with AP dose-dependently reduced an increase of the adhesion of HL-60 cells to TNF-汐-induced HUVEC. Furthermore, we observed that stimulation of TNF-汐 significantly increased intracellular reactive oxygen species (ROS) production. However, pretreatment with AP markedly blocked TNF-汐-induced ROS production in a dose-dependent manner. The western blot and immunofluorescence analysis showed that AP inhibited the translocation of p65 NF-百B to the nucleus. In addition, AP suppressed the TNF-汐-induced degradation of I百B-汐 and attenuated the TNF-汐-induced NF-百B binding. AP also effectively reduced TNF-汐-induced mRNA expressions of monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-8 in a dose-dependent manner. Taken together, AP prevents the vascular inflammatory process through the inhibition of intracellular ROS production and NF-百B activation as well as the reduction of adhesion molecule expression in TNF-汐-induced HUVEC. These results suggested that AP might have a potential therapeutic effect by inhibiting the vascular inflammation process in vascular diseases such as atherosclerosis.
%K Portulaca oleracea
%K inflammation
%K NF-百B
%K reactive oxygen species (ROS)
%K atherosclerosis
%U http://www.mdpi.com/1422-0067/13/5/5628