%0 Journal Article
%T Antimicrobial, Antiproliferative and Proapoptotic Activities of Extract, Fractions and Isolated Compounds from the Stem of Erythroxylum caatingae Plowman
%A Jaciana S. Aguiar
%A Rosilma O. Ara¨²jo
%A Maria do Desterro Rodrigues
%A K¨ºsia X. F. R. Sena
%A Andr¨¦ M. Batista
%A Maria M. P. Guerra
%A Steno L. Oliveira
%A Josean F. Tavares
%A Marcelo S. Silva
%A Silene C. Nascimento
%A Teresinha Gon£¿alves da Silva
%J International Journal of Molecular Sciences
%D 2012
%I MDPI AG
%R 10.3390/ijms13044124
%X In the study, we have examined the antitumor and antimicrobial activities of the methanol extract, the fractions, a fraction of total alkaloids and two alkaloids isolated from the stem of Erythroxylum caatingae Plowman. All test fractions, except the hexane fractions, showed antimicrobial activity on gram-positive bacteria and fungi. The acetate: methanol (95:5), acetate, chloroform and hexane fractions show the highest cytotoxicity activity against the NCI-H292, HEp-2 and K562 cell lines using MTT. The absence of hemolysis in the erythrocytes of mice was observed in these fractions and 6¦Â-Benzoyloxy-3¦Á-(3,4,5-trimethoxybenzoyloxy) tropane (catuabine B). Staining with Annexin V-FITC and JC-1 was used to verify the mechanism of action of the compounds of E. caatingae that showed cytotoxicity less than 30 ¦Ìg/mL in leukemic cells. After 48 h of incubation, we observed that the acetate: methanol (95:5), acetate, and chloroform fractions, as well as the catuabine B, increased in the number of cells in early apoptosis, from 53.0 to 74.8%. An analysis of the potential of the mitochondrial membrane by incorporation of JC-1 showed that most cells during incubation of the acetate: methanol (95:5) and acetate fractions (63.85 and 59.2%) were stained, suggesting the involvement of an intrinsic pathway of apoptosis.
%K Erythroxylum caatingae
%K antimicrobial activity
%K cytotoxic activity
%K hemolytic activity
%K apoptosis
%U http://www.mdpi.com/1422-0067/13/4/4124