%0 Journal Article
%T Mytomycin Made in China Can Be Used to Isolate Embryonic Stem Cells with Ability in Contributing to Germ Lines<br>国产丝裂霉素处理的胎儿成纤维细胞支持具有生殖系转化能力的小鼠胚胎干细胞的分离
%A WEI Zhu-ying
%A SHAO Hua
%A LIU Dong-jun
%A BOU Shor-gan
%A <br>魏著英
%J 动物学研究
%D 2007
%I Chinese Academy of Sciences
%X Application of drugs made in China on culture of embryonic stem cells could decrease the cost of ES cell research. In our experiment, mytomycin made in China(Zhejiang Hisun Pharmaceutical Co.,Ltd, authorized H33020786) was used to process feeder cells, from which mouse embryonic stem cells have been isolated and cultured in vitro. When the blastocysts were cultured directly on treated feeder cell layer, the inner cell masses were growing. After 4-6 days culture, ICM-derived outgrowths were separated into clumps, and placed on mitomycin C-treated mouse embryonic fibroblasts feeder layer. Every 2-3 days, passage were made ever since. These ES cells have been proven to have a potential for germ-line contribution.
%K Mytomycin made in China
%K Mouse Embryonic fibroblasts(MEF)
%K Mouse embryonic stem cells<br>国产丝裂霉素
%K 小鼠胚胎成纤维细胞
%K 小鼠胚胎干细胞
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=44F0567C271B8582D90B3D31395303F6&yid=A732AF04DDA03BB3&vid=D3E34374A0D77D7F&iid=B31275AF3241DB2D&sid=BBA8B1249CDAA6CE&eid=4E8E6A5CE04FD382&journal_id=0254-5853&journal_name=动物学研究&referenced_num=0&reference_num=9