%0 Journal Article
%T Phylogenetic Relationship of the Firefly, Diaphanes pectinealis (Insecta, Coleoptera, Lampyridae) Based on DNA Sequence and Gene Structure of Luciferase<br>栉角雪萤的荧光酶基因结构及其系统发育分析
%A LI Xue-yan
%A YANG Shuang
%A LIANG Xing-cai
%A <br>李学燕
%A 杨爽
%A 梁醒财
%J 动物学研究
%D 2006
%I Chinese Academy of Sciences
%X Diaphanes is the fourth largest genus in Lampyridae, but no luciferase gene from this genus has been reported. In this paper, by PCR amplification of the genomic DNA, the luciferase gene of Diaphanes pectinealis, which is the first case from Diaphanes, was identified and sequenced. The luciferase gene from D. pectinealis spans 1958 base pairs (bp) from the start to the stop codon, including seven exons separated by six introns, and encoding a 547-residuelong polypeptide. Its deduced amino acid sequence showed high protein similarity to those of the Lampyrini tribe (93 -94%) and the Cratomorphini tribe (92%), while low similarity was found with the North American firefly Photinus pyralis (83%) of the Photinini tribe within the same subfamily Lampyrinae. The phylogenetic analysis performed with the deduced amino acid sequences of the luciferase gene further confirms that D. pectinealis, Pyrocoelia, Lampyris, Cratomorphus, and Photinus belong to the same subfamily Lampyrinae, and Diaphanes is closely related to Pyrocoelia, Lampyris,and Cratomorphus. Furthemore, the phylogenetic analysis based on the nucleotide sequences of the luciferase gene indicates Diaphanes is a sister to Lampyris. The phylogenetic analyses are partly consistent with morphological (Branham & Wenzel, 2003) and mitochondrial DNA analyses (Li et al, 2006).
%K Firefly
%K Diaphanes pectinealis
%K Luciferase gene
%K Gene structure
%K Phylogeny<br>栉角雪萤
%K 荧光酶基因
%K 基因结构
%K 系统发育
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=E0BCC7EC1B116A12&yid=37904DC365DD7266&vid=DB817633AA4F79B9&iid=E158A972A605785F&sid=4C2B9916B58305BE&eid=5F8BAECF36EB55E2&journal_id=0254-5853&journal_name=动物学研究&referenced_num=0&reference_num=35