%0 Journal Article
%T Effect of Bovine Serum Albumin on Vitrification of Pronuclear-Stage Mouse Embryo<br>牛血清白蛋白对小鼠原核期胚胎玻璃化冷冻的影响
%A GUAN Mo
%A YANG Shi-hua
%A SI Wei
%A CAI Ke-jun
%A JI Wei-zhi
%A <br>关沫
%A 杨世华
%A 司维
%A 采克俊
%A 季维智
%J 动物学研究
%D 2005
%I Chinese Academy of Sciences
%X The cryoprotective effect and toxicity of Bovine Serum Albumin (BSA) at 8 concentrations (0, 2, 4, 8, 16, 32, 64, 96 mg/mL, respectively) supplemented in cryoprotectant solutions on mouse pronuclear-stage embryo vitrification were studied. Survival rate, cleavage rate, blastocyst rate and mean cell numbers of blastocyst of vitrified embryos were used as criterion to evaluate the effect of BSA on the post-thawed embryos development. The results showed that no statistical differences of survival rate, cleavage rate, blastocyst rate and mean cell numbers of blastocyst of vitrified groups both in the toxic effect assay (P>0.05) and the vitrification assay (P>0.05). The results indicated that BSA in this cryoprotectant solutions has no effect on the viability of pronuclear-stage embryos. Economical, practical and biosecure considerations do not support the use of BSA in vitrificationsolutions.
%K BSA
%K Vitrification
%K Pronuclear-stage embryos
%K Mouse<br>牛血清白蛋白
%K 玻璃化冷冻
%K 原核期胚胎
%K 小鼠
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=AC0CB4AE56D1E53C&yid=2DD7160C83D0ACED&vid=96C778EE049EE47D&iid=0B39A22176CE99FB&sid=2BA123C6EB9D54C2&eid=974CBB04624305A1&journal_id=0254-5853&journal_name=动物学研究&referenced_num=1&reference_num=25