%0 Journal Article
%T Screening and Cloning of RAPD Marker of Fluoride Tolerance Gene in Silkworm,Bombyx mori<br>家蚕耐氟基因RAPD分子标记的筛选及其克隆
%A Abstract
%A <br>徐庆刚
%A 陈克平
%A 姚勤
%A 刘晓勇
%J 动物学研究
%D 2004
%I Chinese Academy of Sciences
%X In this study,silkworm strain T6,tolerant to fluoride,and silkworm strain 733xin,highly sensitive to fluoride,were used to construct the near-isogenic lines.300 random primers were used in RAPD amplification to DNAs of these lines.A molecular marker named S207 was found linked to the fluoride tolerance gene.Examination to F 2 segregated individuals of the above lines verified that this molecular marker was reliable.Subsequently,the molecular marker was cloned into a T vector (pUCm-T) for sequencing.Comparing with sequences available in the GenBank showed that this molecular marker was novel.We plan to convert it into a SCAR marker to facilitate establishment of a molecular marker assisted breeding system.
%K Bombyx mori
%K Near-isogenic lines
%K Fluoride tolerance
%K RAPD marker<br>家蚕耐氟基因
%K RAPD分子标记
%K 筛选
%K 克隆
%K 近等基因系
%K 耐氟性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=6519E86212808C47&yid=D0E58B75BFD8E51C&vid=C5154311167311FE&iid=CA4FD0336C81A37A&sid=CB423C9A71560A74&eid=AA76E167F386B6B3&journal_id=0254-5853&journal_name=动物学研究&referenced_num=0&reference_num=12