%0 Journal Article
%T Assessment of Homozygosity in Gynogenetic Diploid Using Microsatellite Markers in Japanese Flounder (Paralichthys olivaceus)<br>微卫星评价牙鲆雌核发育二倍体纯合性
%A ZHU Xiao-chen
%A LIU Hai-jin
%A SUN Xiao-wen
%A XUE Ling-ling
%A MAO Lian-ju
%A <br>朱晓琛
%A 刘海金
%A 孙效文
%A 薛玲玲
%A 毛连菊
%J 动物学研究
%D 2006
%I Chinese Academy of Sciences
%X Gynogenesis was thought to be a useful method to generate inbred lines in fish. In this study, analysis of 5-8 heterozygous loci in Japanese flounder (Paralichthys olivaceus) was used to measure the increment of homozygosity in meiogynogenetic diploid (Meiotic-A & Meiotic-B) and mitogynogenetic diploid (Mitotic-A). The result showed all homozygosity in Mitotic-G1, while a high percentage of heterozygosity in Meiosis-A and Meiosis-B except Poli11TUF. The rate of heterozygosity in meiogynogenetic progenies at the locus of Poli9TUF, Poli9-8TUF, Poli11TUF, Poli13TUF, Poli23TUF, Poli30TUF, Poli123TUF and Poli130TUF were 1.000 0, 1.000 0, 0.194 4, 0.945 9, 0.861 1, 1.000 0, 0.777 8 and 0.800 0, respectively. The average recombination rate of these eight loci is 0.822 4. The high proportion of heterozygotes for seven loci demonstrates that it is not a practical method for producing homozygous inbred lines in the gynogenetic fish produced by retention the second polar body; and treatments of suppressing the first cell division were more promising for this purpose.
%K Japanese flounder
%K Gynogenesis
%K Homozygosity
%K Microsatellite marker<br>牙鲆
%K 雌核发育
%K 纯合性
%K 微卫星
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=E674180475056C2A&yid=37904DC365DD7266&vid=DB817633AA4F79B9&iid=CA4FD0336C81A37A&sid=E84BBBDDD74F497C&eid=5D71B28100102720&journal_id=0254-5853&journal_name=动物学研究&referenced_num=18&reference_num=20