%0 Journal Article
%T Molecular Cloning and Comparison of Ubiquitin Fusion Protein and Ribosomal Protein L30 from Ophiophagus hannah<br>眼镜王蛇泛素融合蛋白基因和核糖体蛋白L30基因的克隆及分析
%A WEI Shuang-shuang
%A ZHANG Ying-xia
%A LEE Wen-hui
%A ZHANG Yun
%A <br>韦双双
%A 张英霞
%A 李文辉
%A 张云
%J 动物学研究
%D 2005
%I Chinese Academy of Sciences
%X Total RNA was extracted from the venom gland of snake Ophiophagus hannah. A king cobra venom gland cDNA library was then constructed using purified mRNA from the total RNA. By random sequencing of 200 independent clones, two conserved cDNA sequences of house-keeping genes were obtained. One is ubiquitin fusion protein (GenBank accession number AF297036); the other is ribosomal protein L30 (GenBank accession number AF297033). The former has an open reading frame of 387 bp, encoding a 128 amino acid ubiquitin fusion protein precursor composed of a 76 amino acid ubiquitin domain and followed by a 52 amino acid ribosomal protein L40 domain. The deduced precursor protein from the nucleotide sequence is basic. The C-terminus of the ubiquitin fusion protein contains the zinc-finger motif. The latter has an open reading frame of 348 bp, encoding for 115 amino acid ribosomal protein L30 precursor. The predicted amino acid sequence of ubiquitin fusion protein is highly conserved when compared to the sequences of homologous proteins from sixteen diverse species.
%K Ophiophagus hannah
%K Ubiquitin fusion protein
%K Carboxyl-extension protein
%K Ribosomal protein<br>眼镜王蛇
%K 泛素融合蛋白
%K C-末端延伸蛋白
%K 核糖体蛋白
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=667D1641013FE2C4&yid=2DD7160C83D0ACED&vid=96C778EE049EE47D&iid=E158A972A605785F&sid=21D8CE17EE5EE354&eid=1E9426A299DC9FFD&journal_id=0254-5853&journal_name=动物学研究&referenced_num=2&reference_num=11