%0 Journal Article
%T Cloning and Sequencing of the Sox Genes in Chinemys reevesii<br>乌龟Sox基因的克隆及测序
%A 张海军
%A 聂刘旺
%J 动物学研究
%D 2001
%I Chinese Academy of Sciences
%X Using a pair of degenerate primers based on the conservative region,HMG-box,of human SRY gene,four different fragments were amplified from both female and male Chinemys reevesii,then cloned by using pGEM-T vector and sequenced.The sequence result indicated their high homology to human Sox genes.The identities to human Sox genes in the DNA sequence and the amino acid sequence are 92%,91%,84%,92% and 100%,93%,98%,98%,respectively.It might be concluded that Sox gene was highly conservative in phylogenesis.Our work provides molecular data for the study of sex-determining mechanism of C.reevesii,which might be temperature-dependent sex determination.
%K Chinemys reevesii
%K Sox gene
%K Cloning
%K Sequencing<br>乌龟
%K Sox基因
%K 克隆
%K 测序
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=5DF5E4C00CAA3A24&yid=14E7EF987E4155E6&vid=BC12EA701C895178&iid=E158A972A605785F&sid=50EA2A80A7D254EF&eid=5A6705FDACED0BF9&journal_id=0254-5853&journal_name=动物学研究&referenced_num=6&reference_num=15