%0 Journal Article
%T Function of Sugars During Cryopreservation of Rhesus Macaque (Macaca mulatta) Spermatozoa<br>糖在猕猴精子低温冷冻保存过程中的作用
%A LI Xi long
%A SI Wei
%A WANG Hong
%A ZOU Ru jin
%A JI Wei zhi
%A <br>李喜龙
%A 司维
%J 动物学研究
%D 2002
%I Chinese Academy of Sciences
%X This study was conducted to evaluate the cryoprotective function of sugars in two freezing media (TTE and TEST) by comparing the spermatozoa morphologic change of rhesus monkey before and after cryopreservation using transmission electron microscopy. The results showed that the structure of acrosome integrity, nuclear and tail of most spermatozoa cryopreserved with TTE was similar with that of fresh spermatozoa, except for the slight swell of plasma membrane. However, in spermatozoa cryopreserved with TEST, the plasma membrane were fluctuated, swelled and broken, the acrosome integrity and nuclear were damaged, and the structure of tail were changed significantly. It demonstrated that TTE is a suitable freezing media for rhesus monkey spermatozoa cryopreservation. Sugars exert cryoprotective roles by protecting the intact of plasma membrane and acrosome integrity, but the protective roles of sugars are significant different.
%K Rhesus macaque
%K Spermatozoa
%K Cryopreservation
%K Ultrastructure<br>猕猴精子
%K 低温冷冻保存
%K 作用
%K 糖类
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=CCB6B916F40ABE1A&yid=C3ACC247184A22C1&vid=EA389574707BDED3&iid=38B194292C032A66&sid=EF27C460877D3C9F&eid=1B07C0EDADD830201D2CBF3B1D560B30&journal_id=0254-5853&journal_name=动物学研究&referenced_num=4&reference_num=25