%0 Journal Article
%T A SANDWICH ENZYME-IMMUNOASSAY FOR DETECTING SNAKE VENOM<br>酶标免疫分析法鉴定蛇毒
%A Ge Bing Yu Huiqun Wang Jingying
%A <br>葛兵
%A 许燕
%J 动物学研究
%D 1991
%I Chinese Academy of Sciences
%X The rapid identification of snake venom in snakebite is very helpful to the treatment of the snakebite. We have purified three kinds of antibody Fab of snake venoms by affinity chromatography on different immobilized snake venom, in order to avoid crossimmunoreaction. Horseradish peroxidase was coupled to these Fab fragments by the periodate method. We have developed a sandwich enzyme-immunoassay, which is capable of detecting < 5 ng of snake venom in about 90 minutes. This assay used in clinical identification of snake venom has achieved satisfied results.
%K Snake Venom
%K Snakebite
%K Enzyme-immunoassay<br>蛇毒类
%K 蛇咬伤
%K 免疫酶技术
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=B9408D4B3DCE12DE&yid=116CB34717B0B183&vid=59906B3B2830C2C5&iid=CA4FD0336C81A37A&sid=9C65ADEB5990B252&eid=06EA2770E96C5402&journal_id=0254-5853&journal_name=动物学研究&referenced_num=0&reference_num=2