%0 Journal Article
%T Physical Blocking Neural Tube Closure Affects Radial Intercalation and Neural Crest Midline-directed Migration in Xenopus Dorsal Explants<br>机械阻断神经管的闭合影响爪蟾神经管辐射状切入及神经嵴细胞定向迁移(英文)
%A SHI Yu
%A ZHAO Shu-hua
%A MAO Bing-yu
%A <br>石宇
%A 赵树华
%A 毛炳宇
%J 动物学研究
%D 2009
%I Chinese Academy of Sciences
%X Neural tube defects (NTDs) are severe congenital malformation diseases, which occur in 1 out of 1000 births in human. In Xenopus, several tissue movements are involved in the neural tube closure process. Immediately after the neural tube fusion, the neural crest cells get monopolar protrusion toward dorsal midline and migrate to form the roof of the neural tube. At the same time, radial intercalation takes place from the ventral neural tube and forces it to be single-layered. Here, we physically block the neural tube closure to test the cell movements and the following patterning in Xenopus laevis explants. The results show that the single-layered neural tube fails to form and the neural crest cells remain at the lateral regions in the explants with NTDs. However, the patterning of the neural tube is not affected as indicated by the normal expression of the preneural genes. These results indicate a requirement of the neural tube fusion for the radial intercalation and the dorsal midline directed neural crest migration, but not for the dorsal-ventral patterning of the neural tube.
%K Xenopus laevis
%K Neural tube defect
%K Neural tube closure
%K Neural crest
%K Morghogenesis
%K Intercalation
%K Dorsoventral patterning<br>非洲爪蟾
%K 神经管缺陷
%K 神经管闭合
%K 神经嵴
%K 形态发生
%K 细胞切入
%K 背腹图式
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=D179322949C7EB0C24BE5EBC1C746208&yid=DE12191FBD62783C&vid=340AC2BF8E7AB4FD&iid=B31275AF3241DB2D&sid=46C2A519EDDA03DD&eid=0636354D8CF77519&journal_id=0254-5853&journal_name=动物学研究&referenced_num=0&reference_num=0