%0 Journal Article
%T Expression of the Ghrelin in Sheep Ooctyes and Pre- implantation Embryos Produced in vivo<br>Ghrelin在绵羊体内卵母细胞和早期胚胎的表达
%A DU Chen-guang
%A YANG Yan-yan
%A LI Hai-jun
%A BAO Qing-jiang
%A GUO Hong-ru
%A CAO Gui-fang
%A <br>杜晨光
%A 杨燕燕
%A 李海军
%A 鲍庆江
%A 郭宏儒
%A 曹贵方
%J 动物学研究
%D 2008
%I Chinese Academy of Sciences
%X The possibility of ghrelin expressed in sheep oocytes and pre-implantation embryos produced in vivo was investigated in this study. The observed ghrelin immunoreactivity of oocytes and embryos at all stages was predominantly in the cytoplasm. Relative real-time reverse-transciptase (RT)-PCR experiments confirmed that the ghrelin mRNA levels varied depending on the developmental stage, with the highest expression in the blastocyst, metaphase II (MII) oocytes, 2- and 8-cell stages had a significantly higher expression in the germinal vesicle (GV) oocytes, 4-stage and morula. Dynamic changes and the persistent presence of the ghrelin signaling system within oocytes and pre-implantation embryos opens up the possibility of a potential regulatory role of this novel molecule during oocyte maturation and embryonic development.
%K Ghrelin
%K Sheep oocytes
%K Embryos
%K Real time RT-PCR
%K Immunofluorescence staining<br>Ghrelin
%K 绵羊卵母细胞
%K 胚胎
%K 实时定量RT-PCR
%K 免疫荧光染色
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=611DF3B2A7637214999BF970BB97AE9C&yid=67289AFF6305E306&vid=771469D9D58C34FF&iid=0B39A22176CE99FB&sid=03A030BB0C519C60&eid=09E495F616948E78&journal_id=0254-5853&journal_name=动物学研究&referenced_num=1&reference_num=15