%0 Journal Article
%T Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting<br>利用相互染色体涂色技术分析两株人肺腺癌细胞系(A549和GLC-82)的核型特征
%A PENG Kun-Jing
%A WANG Jin-Huan
%A SU Wei-Ting
%A WANG Xi-Cai
%A YANG Feng-Tang
%A NIE Wen-Hui
%A <br>彭昆靖
%A 王金焕
%A 苏伟婷
%A 王熙才
%A 杨凤堂
%A 佴文惠
%J 动物学研究
%D 2010
%I Chinese Academy of Sciences
%X Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established from patients induced by different factors, a combined approach of chromosome sorting, forward and reverse chromosome painting was used to characterize karyotypes of two lung adenocarcinoma cell lines: A549 and GLC-82 with the latter line derived from a patient who has suffered long-term exposure to environmental radon gas pollution. The chromosome painting results revealed that complex chromosomal rearrangements occurred in these two lung adenocarcinoma cell lines. Thirteen and twenty-four abnormal chromosomes were identified in A549 and GLC-82 cell lines, respectively. Almost half of abnormal chromosomes in these two cell lines were formed by non-reciprocal translocations, the others were derived from deletions and duplication/or amplification in some chromosomal regions. Furthermore, two apparently common breakpoints, HSA8q24 and 12q14 were found in these two lung cancer cell lines.
%K Lung adenocarcinoma cell lines
%K Chromosome sorting
%K Chromosome painting
%K Cytogenetic characterization<br>肺腺癌细胞系
%K 染色体分选
%K 染色体涂色
%K 细胞遗传学特征
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=5B533C5A37AABE3FBDD8C4DDF67B4F90&yid=140ECF96957D60B2&vid=4AD960B5AD2D111A&iid=0B39A22176CE99FB&sid=A63576421B012172&eid=CDEBD1ACE0A4C1C1&journal_id=0254-5853&journal_name=动物学研究&referenced_num=0&reference_num=35