%0 Journal Article
%T Differential Proteomics Identification of HSP90 as Potential Serum Biomarker in Hepatocellular Carcinoma by Two-dimensional Electrophoresis and Mass Spectrometry
%A Yiyi Sun
%A Zhihe Zang
%A Xiaohong Xu
%A Zhonglin Zhang
%A Ling Zhong
%A Wang Zan
%A Yan Zhao
%A Lin Sun
%J International Journal of Molecular Sciences
%D 2010
%I MDPI AG
%R 10.3390/ijms11041423
%X The aim of the current study is to identify the potential biomarkers involved in Hepatocellular carcinoma (HCC) carcinogenesis. A comparative proteomics approach was utilized to identify the differentially expressed proteins in the serum of 10 HCC patients and 10 controls. A total of 12 significantly altered proteins were identified by mass spectrometry. Of the 12 proteins identified, HSP90 was one of the most significantly altered proteins and its over-expression in the serum of 20 HCC patients was confirmed using ELISA analysis. The observations suggest that HSP90 might be a potential biomarker for early diagnosis, prognosis, and monitoring in the therapy of HCC. This work demonstrates that a comprehensive strategy of proteomic identification combined with further validation should be adopted in the field of cancer biomarker discovery.
%K proteomics
%K hepatocellular carcinoma
%K serum biomarker
%U http://www.mdpi.com/1422-0067/11/4/1423