%0 Journal Article %T Differential Proteomics Identification of HSP90 as Potential Serum Biomarker in Hepatocellular Carcinoma by Two-dimensional Electrophoresis and Mass Spectrometry %A Yiyi Sun %A Zhihe Zang %A Xiaohong Xu %A Zhonglin Zhang %A Ling Zhong %A Wang Zan %A Yan Zhao %A Lin Sun %J International Journal of Molecular Sciences %D 2010 %I MDPI AG %R 10.3390/ijms11041423 %X The aim of the current study is to identify the potential biomarkers involved in Hepatocellular carcinoma (HCC) carcinogenesis. A comparative proteomics approach was utilized to identify the differentially expressed proteins in the serum of 10 HCC patients and 10 controls. A total of 12 significantly altered proteins were identified by mass spectrometry. Of the 12 proteins identified, HSP90 was one of the most significantly altered proteins and its over-expression in the serum of 20 HCC patients was confirmed using ELISA analysis. The observations suggest that HSP90 might be a potential biomarker for early diagnosis, prognosis, and monitoring in the therapy of HCC. This work demonstrates that a comprehensive strategy of proteomic identification combined with further validation should be adopted in the field of cancer biomarker discovery. %K proteomics %K hepatocellular carcinoma %K serum biomarker %U http://www.mdpi.com/1422-0067/11/4/1423