%0 Journal Article
%T Resveratrol Inhibited Hydroquinone-Induced Cytotoxicity in Mouse Primary Hepatocytes
%A Da-Hong Wang
%A Yoshie Ootsuki
%A Hirofumi Fujita
%A Masahiro Miyazaki
%A Qinxia Yie
%A Ken Tsutsui
%A Kuniaki Sano
%A Noriyoshi Masuoka
%A Keiki Ogino
%J International Journal of Environmental Research and Public Health
%D 2012
%I MDPI AG
%R 10.3390/ijerph9093354
%X Hydroquinone (1,4-benzenediol) has been widely used in clinical situations and the cosmetic industry because of its depigmenting effects. Most skin-lightening hydroquinone creams contain 4%¨C5% hydroquinone. We have investigated the role of resveratrol in prevention of hydroquinone induced cytotoxicity in mouse primary hepatocytes. We found that 400 ¦ÌM hydroquinone exposure alone induced apoptosis of the cells and also resulted in a significant drop of cell viability compared with the control, and pretreatment of resveratrol to a final concentration of 0.5 mM 1 h before hydroquinone exposure did not show a significant improvement in the survival rate of the hepatocytes, however, relatively higher concentrations of resveratrol (¡Ý1 mM) inhibited apoptosis of the mouse primary hepatocytes and increased cell viability in a dose-dependent manner, and in particular the survival rate of the hepatocytes was recovered from 28% to near 100% by 5 mM resveratrol. Interestingly, pretreatment with resveratrol for longer time (24 h), even in very low concentrations (50 ¦ÌM, 100 ¦ÌM), blocked the damage of hydroquinone to the cells. We also observed that resveratrol pretreatment suppressed hydroquinone-induced expression of cytochrome P450 2E1 mRNA dose-dependently. The present study suggests that resveratrol protected the cells against hydroquinone-induced toxicity through its antioxidant function and possibly suppressive effect on the expression of cytochrome P450 2E1.
%K resveratrol
%K hydroquinone
%K cytotoxicity prevention
%K CYP 2E1
%K primary culture of mouse hepatocytes
%U http://www.mdpi.com/1660-4601/9/9/3354